Improved Ruthenium II tris (bathophenantroline disulfonate) staining and destaining protocol for a better signal‐to‐background ratio and improved baseline resolution
- 19 February 2004
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 4 (3) , 599-608
- https://doi.org/10.1002/pmic.200300587
Abstract
In proteomics the ability to visualize proteins from electropherograms is essential. Here a new protocol for staining and destaining gels treated with Ruthenium II tris (bathophenantroline disulfonate) is presented. The method is compared with the silver-staining procedure of Swain and Ross, the Ruthenium II tris (bathophenantroline disulfonate) stain described by Rabilloud (Rabilloud T., Strub, S. M. Luche, S., Girardet, S. L. et al., Proteomics 2001, 1, 699–704) and the SYPRO Ruby gel stain. The method offers a better signal-to-background ratio with improved baseline resolution for both sodium dodecyl sulfate-polyacrylamide gels and two-dimensional gels.Keywords
This publication has 12 references indexed in Scilit:
- An improved formulation of SYPRO Ruby protein gel stain: Comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulationProteomics, 2002
- The rat liver mitochondrial proteinsElectrophoresis, 2002
- Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysisProteomics, 2001
- A comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gelsProteomics, 2001
- Comparison of three different fluorescent visualization strategies for detectingEscherichia coli ATP synthase subunits after sodium dodecyl sulfate-polyacrylamide gel electrophoresisProteomics, 2001
- Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complexElectrophoresis, 2000
- Reliable automatic protein identification from matrix-assisted laser desorption/ionization mass spectrometric peptide fingerprintsElectrophoresis, 1999
- A Luminescent Ruthenium Complex for Ultrasensitive Detection of Proteins Immobilized on Membrane SupportsAnalytical Biochemistry, 1999
- Identification of Proteins by Matrix-Assisted Laser Desorption Ionization–Mass Spectrometry Following In-Gel Digestion in Low-Salt, Nonvolatile Buffer and Simplified Peptide RecoveryAnalytical Biochemistry, 1997
- A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gelsElectrophoresis, 1995