Restriction enzyme mapping of the nuclear ribosomal cistron in selected Laminariales (Phaeophyta): a phylogenetic assessment

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Abstract
Restriction enzyme mapping of the nuclear ribosomal cistron was completed for a variety of Laminariales. Taxa investigated included Alaria marginata Postels and Ruprecht, Egregia menziesii (Turner) Areschoug, Eisenia arborea Areschoug, Lessoniopsis littoralis (Tilden) Reinke, Macrocystis integrifolia Bory, Nereocystis leutkeana (Mertens) Postels and Ruprecht, Postelsia palmaeformis Ruprecht, and Pterygophora californica Ruprecht, with Sargassum muticum (Yendo) Fensholt (Fucales) as an outgroup. The restriction maps establish a foundation for future phylogenetic, as well as other molecular, investigations in the kelp. We also assess restriction enzyme mapping of the nuclear ribosomal cistron for suitability in resolving intrafamilial and interfamilial taxonomic relationships in the Laminariales. The intergenic spacer proved too variable to be of use for phylogenetic comparisons at this level. Conversely, the gene regions were highly conserved, with only three restriction-site differences observed among all the laminarialean taxa investigated. Key words: Laminariales, rRNA, restriction enzyme mapping.

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