Antibodies to N-homocysteinylated albumin as a marker for earlyonset coronary artery disease in men
Open Access
- 1 January 2005
- journal article
- Published by Georg Thieme Verlag KG in Thrombosis and Haemostasis
- Vol. 93 (02) , 346-350
- https://doi.org/10.1160/th04-08-0493
Abstract
N-homocysteinylated (Nε-Hcy) proteins and corresponding antibodies have recently been discovered in humans and animals. Increased autoimmune response to Nε -Hcy-proteins has been reported in stroke patients. The aim of the present study was to investigate whether antibodies against N-homocysteinylated albumin are associated with coronary artery disease (CAD).We studied 88 male patients aged 50 years or under with angiographically documented CAD and 100 age-matched apparently healthy men as controls. Serum levels of IgG antibodies against Nε-Hcy-albumin were determined using an enzymelinked immunosorbent assay. Seropositivity to anti-Nε -Hcy-albumin antibodies was 5-fold more frequent in CAD patients than in controls (52.3 % vs 10.0 %; p14.5 mM were seropositive compared with 25.5 % of normohomocysteinemic individuals (p=0.048).There was a weak correlation between anti-Nε-Hcy-albumin antibodies and Hcy levels (r=0.16; p=0.03). By multivariate logistic regression analysis, seropositivity to anti-Nε-Hcy-albumin antibodies was an independent predictor of early CAD (OR, 14.82; 95% CI, 4.47 to 49.19; p=0.00002). Interestingly, anti-Nε-Hcy-albumin antibodies were associated with C-reactive protein levels (r=0.24; p=0.002). Seropositivity to anti-Nε-Hcy-albumin antibodies showed no association with the MTHFR C677T polymorphism. Our results suggest that seropositivity to antibodies against Nε-homocysteinylated albumin is associated with early-onset CAD. An autoimmune response to Nε-Hcy-albumin may represent a novel mechanism involved in the early development of CAD.Keywords
Funding Information
- American Heart Association ((to H. J.))
This publication has 26 references indexed in Scilit:
- Autoantibodies Against N -Homocysteinylated Proteins in HumansStroke, 2004
- Cross-talk between Cys34 and Lysine Residues in Human Serum Albumin Revealed by N-HomocysteinylationJournal of Biological Chemistry, 2004
- Moderate hyperhomocysteinaemia and immune activation in Parkinson's diseaseJournal Of Neural Transmission-Parkinsons Disease and Dementia Section, 2002
- Innate and acquired immunity in atherogenesisNature Medicine, 2002
- Homocysteine Is a Protein Amino Acid in HumansJournal of Biological Chemistry, 2002
- Immune Mechanisms in AtherosclerosisArteriosclerosis, Thrombosis, and Vascular Biology, 2001
- Mutation A1298C of methylenetetrahydrofolate reductase: Risk for early coronary disease not associated with hyperhomocysteinemiaAmerican Journal of Medical Genetics, 2001
- Homocysteine — an innocent bystander in vascular disease?Published by Oxford University Press (OUP) ,2001
- Homocysteine and coronary atherosclerosisJournal of the American College of Cardiology, 1996
- A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductaseNature Genetics, 1995