Expression in a wine yeast strain of theAspergillus niger abfBgene

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Abstract
A recombinant wine yeast strain has been constructed expressing the gene coding for α-l-arabinofuranosidase B from Aspergillus niger under the control of the yeast actin gene promoter. The protein is efficiently secreted by the recombinant yeast, allowing its purification and characterisation. The heterologous α-l-arabinofuranosidase B shows similar physico-chemical properties to the native enzyme. The wine produced in microvinification experiments using the recombinant yeast presents the same oenological characteristics as obtained with the untransformed strain. The α-L-arabinofuranosidase B protein is detected throughout the fermentation.