Isolation, Purification, and Partial Characterization of Formate Dehydrogenase from Soybean Seed
- 1 November 1983
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 73 (3) , 858-859
- https://doi.org/10.1104/pp.73.3.858
Abstract
Soybean (Glycine soja var Beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. The enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. It has activity over a broad pH range, is stable for months at 4 degrees C, and has K(m) values of 0.6 millimolar and 5.7 micromolar for formate and NAD, respectively.Keywords
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