SUPPRESSION OF A LAC O° MUTATION IN ESCHERICHIA COLI

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Abstract
Schwartz, Norman M. (Yale University, New Haven, Conn.). Suppression of a lac o° mutation in Escherichia coli . J. Bacteriol. 88: 996–1001. 1964.—A class of pro – markers which can be cotransduced with lac map close to the i side of the lac region. The relative order of markers transferred by Hfr H is thr leu proA proB lac proC ade . Nine of ten slowgrowing lactose-utilizing revertants of a lac o° mutant are suppressed mutants. The time of entry of su-lac o-5 from an Hfr H derivative is 27 min. Su-lac o-5 is separated from the lac region by a 10-min interval. Su-lac o-5 is unstable, restores 7% of the wild-type level of β-galactosidase activity, suppresses a try – mutation, and has a deleterious effect upon growth rate. The primary lac o° mutation is interpreted as being a z – polarity mutation which constitutes nonsense. Su-lac o-5 could act at the level of messenger ribonucleic acid translation converting nonsense to sense by promoting mistakes in protein synthesis.

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