Biochemical and Histochemical Studies of Aerobic Oxidative Metabolism of Oral Tissues. IV. Additional Observations on Histochemical Oxidase Reactions in Gingiva

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Abstract
The "G-Nadi" and Burstone procedures for oxidase localization in human and dog gingiva were compared. Both methods indicated highest oxidase activity in epithelial attachment and crevicular areas; N, N dimethyl-p-phenylene diamine without coupling agents effectively localized oxidase activity. An insoluble green dye which is described for the first time is produced in the tissues in areas of low activity. The green color rapidly changes to a deep blue in areas of high activity. The original "G-Nadi" and Burstone procedures usually give similar results except that parakeratin and keratin also exhibit color formation when incubation time is more than one hour in the former method. Extraction of sections of gigival tissue with distilled water or petroleum ether prior to osidase localization in both procedures, significantly activated color formation. The metabolism of different gingival tissue components was discussed in relation to alterations in chemical components of gingiva during the inflammatory process.