Enzymatic synthesis of poly-?-hydroxybutyrate inZoogloea ramigera
- 1 November 1976
- journal article
- research article
- Published by Springer Nature in Archiv für Mikrobiologie
- Vol. 110 (2-3) , 149-156
- https://doi.org/10.1007/bf00690222
Abstract
The enzyme activity synthesizing poly-β-hydroxybutyrate (PHB) was mainly localized in the PHB-containing particulate fraction ofZoogloea ramigera I-16-M, when it grew flocculatedly in a medium supplemented with glucose. On the other hand, the enzyme activity remained in the soluble fraction, when the bacterium grew dispersedly in a glucose-starved medium. The soluble PHB synthase activity became associated with the particulate fraction as PHB synthesis was initiated on the addition of glucose to the dispersed culture. Conversely, the enzyme activity was released from the PHB-containing granules to the soluble fraction when the flocculated culture was kept incubated without supplementing the medium with glucose. PHB synthase was also incorporated into the newly formed PHB fraction when partially purified soluble PHB synthase was incubated withd(-)-β-hydroxybutyryl CoA in vitro. Although attempts to solubilize the particulate enzyme were unsuccessful, and the soluble enzyme became extremely unstable in advanced stages of purification, both PHB synthases had the same strict substrate specificity ford(-)-β-hydroxybutyryl CoA, and showed the same pH optimum at 7.0.Keywords
This publication has 16 references indexed in Scilit:
- The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckiiBiochemical Journal, 1973
- The role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckiiBiochemical Journal, 1972
- Metabolism of poly(β-hydroxybutyrate). I. Purification, composition, and properties of native poly(β-hydroxybutyrate) granules from Bacillus megateriumBiochemistry, 1968
- Zoogloea ramigera Itzigsohn, identification and descriptionInternational Journal of Systematic and Evolutionary Microbiology, 1967
- Cyst Formation and Poly-β-Hydroxybutyric Acid Accumulation in AzotobacterJournal of Bacteriology, 1966
- SOME ASPECTS OF THE ENDOGENOUS METABOLISM OF BACTERIAMicrobiology and Molecular Biology Reviews, 1964
- Enzymatic Synthesis of Poly-β-Hydroxybutyric Acid in BacteriaNature, 1961
- A Rapid Spectrophotometric Assay of Alpha, Beta-Unsaturated Acids and Beta-Hydroxy AcidsAnalytical Chemistry, 1960
- Synthese von S‐β‐Oxybutyryl‐ und S‐Acetacetyl‐Coenzym AAngewandte Chemie, 1953
- Studies of Sewage Purification: II. A Zooglea-Forming Bacterium Isolated from Activated SludgePublic Health Reports (1896-1970), 1935