A Mems-based Miniature DNA Analysis System
- 1 January 1995
- conference paper
- Published by Institute of Electrical and Electronics Engineers (IEEE)
- Vol. 40, 764-767
- https://doi.org/10.1109/sensor.1995.717344
Abstract
In this report we detail the design and development of a miniature thermal cycling instrument for performing the polymerase chain reaction (PCR) that uses microfabricated, silicon-based reaction chambers. The MEMS- based, battery-operated instrument shows significant improvements over commercial thermal cycling instrumentation. Several different biological systems have been amplified and verifyed with the miniature PCR instrument including the Human Immunodeficiency Virus (HIV); both cloned and genomic DNA templates of /spl beta/ globin; and the genetic disease, Cystic Fibrosis (CF) from human DNA. The miniaturization of a PCR thermal cycler is the initial module of a fully- integrated portable, low-power, rapid, and highly efficient bioanalytical instrument.Keywords
This publication has 3 references indexed in Scilit:
- µ-TAS: Miniaturized Total Chemical Analysis SystemsPublished by Springer Nature ,1995
- PCR in a silicon microstructureClinical Chemistry, 1994
- Minimizing the time required for DNA amplification by efficient heat transfer to small samplesAnalytical Biochemistry, 1990