Lipid extracting properties of 2,2-dimethoxypropane as revealed by electron microscopy and thin layer chromatography
- 1 January 1982
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 76 (3) , 407-412
- https://doi.org/10.1007/bf00543960
Abstract
Dehydration with 2,2-dimethoxypropane acidified with either 1 N HCL or 0.05 N HCL in an 1:100 ratio has been found to extract various biologically active lipids. The effect is enhanced by an increased proton concentration and seems to occur only after complete H2O transformation. It is suggested that lipid extraction is caused by a reoxidation of lower osmium oxides with subsequent attack on the unfixed lipids by the organic solvent DMP.This publication has 3 references indexed in Scilit:
- Problems of the use of 2,2-dimethoxypropane as dehydrating agent in preparing single cells for transmission electron microscopyHistochemistry and Cell Biology, 1982
- Optimization and investigation of the use of 2,2-dimethoxypropane as a dehydration agent for plant tissues in transmission electron microscopyJournal of Ultrastructure Research, 1979
- Rapid chemical dehydration of samples for electron microscopic examinations.Journal of Histochemistry & Cytochemistry, 1975