A simple and reliable method of chromosome banding for prenatal cytogenetics using a bromodeoxyuridine pulse

1 October 1983

journal article
research article
Published by Wiley in Prenatal Diagnosis

Vol. 3 (4) , 291-296
https://doi.org/10.1002/pd.1970030405

Abstract

An extremely simple, fully defined and reliable technique for banding chromosomes in situ is described. The method uses a pulse of bromodeoxyuridine to produce replication pattern banding complete with G‐ and C‐type banding in the same karyotype. This enables immediate resolution of problems otherwise requiring extra subculturing and the application of conventional banding techniques. The value of routine chromosome banding in prenatal cytogenetics using such an economical technique is discussed.

Keywords

This publication has 10 references indexed in Scilit:

High resolution R- and G-banding on the same preparation
Human Genetics, 1981
Pattern of chromosomal replication in synchronized lymphocytes
Human Genetics, 1980
Utility and limitations of chromosome banding in pre- and postnatal service cytogenetics
American Journal of Medical Genetics, 1980
Demonstration of replication patterns corresponding to G- and R-type banding of chromosomes after partial sunchronization of cell cultures with BrdU or dT surplus
Human Genetics, 1978
Analysis of DNA replication patterns of human fibroblast chromosomes the replication map
Human Genetics, 1978
ISCN 1978
Cytogenetic and Genome Research, 1978
DNA replication patterns of the early S phase from amniotic fluid cells as revealed by a Giemsa staining technique
Human Genetics, 1976
A RAPID BANDING TECHNIQUE FOR HUMAN CHROMOSOMES
The Lancet, 1971
New Technique for Distinguishing between Human Chromosomes
Nature New Biology, 1971
The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability
Hereditas, 1971