Callus was induced from isolated gladiolus cormel stem tips on modified Murashige and Skoog media. The callus was then induced to differentiate virus-symptomless plants with corms. Morphogenesis from the callus included meristematic zones on or within the callus, organoids with vascular tissue, stems, leaves, and plants with roots. Callus production and differentiation of plants were more frequent on agar than in liquid media.