De-Semination of Fertilized Drosophila melanogaster Females

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Abstract
De-semination of fertilized D. melanogaster females is easily achieved using a standard household refrigerator without any special equipment. The exposure for optimal results seems to differ with the strain employed. The duration of treatment adapted for the strains used was 50 minutes at -5[degree]C, with a mortality of 50% in one strain and 25% in the other one. The influence of the treatments on hatchability of eggs laid by treated and subsequently remated females varies according to the strain. The treatment does not alter the level of larval and pupal survival or fertility of the progeny of treated females, when compared with that of untreated ones.

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