Deoxyribonucleic Acid Homology and Other Comparisons among Obligately Thermophilic Hydrocarbonoclastic Bacteria, with a Proposal for Thermoleophilum minutum sp. nov.

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Abstract
Bacterial strains in our culture collection similar to the recently proposed species of Thermoleophilum album were examined for generic relatedness. We compared these obligately thermophilic hydrocarbonoclastic bacteria by determining the electrophoretic patterns of enzymes and soluble proteins and by using deoxyribonucleic acid-deoxyribonucleic and hybridization techniques. Included in the hybridization study together with these strains were members of the genera Thermus, Thermomicrobium, and Bacillus. Our results confirmed that Thermoleophilum is a valid genus since no measurable reassociation was observed with the members of the other genera examined. There are two homology groups within the genus Thermoleophilum; T. album strain HS-5T (= ATCC 35263T) (T = type strain) represents the type species of the genus, and Thermoleophilum minutum sp. nov. is a second species proposed here. The type strain of T. minutum is strain YS-4 (= ATCC 35265).