Curing of the killer deoxyribonucleic acid plasmids of Kluyveromyces lactis

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Abstract
UV irradiation gave rise to frequent curing of killer plasmids pGKl1 and pGKl2 of K. lactis. Almost all of the nonkillers obtained lost both plasmids, but 1 lost only pGKl1. The disappearance of pGKl1 was accompanied by the simultaneous loss of the killer activity and of the resistance to the killer factor. A new plasmid, pGKl1S, was obtained, which arose from a deletion in the central region of pGKl1. Genetic analysis suggested that pGKl1S has the killer gene lost by the deletion and the resistance gene intact and that pGKl1S shares the same replication control with pGKl1.