Detection of Rotavirus in South African Waters: A Comparison of a Cytoimmunolabelling Technique with Commercially Available Immunoassays
- 1 July 1991
- journal article
- Published by IWA Publishing in Water Science & Technology
- Vol. 24 (2) , 241-244
- https://doi.org/10.2166/wst.1991.0066
Abstract
A cytoimmunolabelling technique was compared with commercially available immunoassays for the detection of the simian rotavirus SA11 and human rotavirus in various environmental samples. The technique is based on labelling MA104 cells with antibody conjugated with an enzyme for the detection of rotavirus. Water samples were concentrated by ultrafiltration and inoculated on trypsin-treated MA104 cells. After 18 h incubation, evidence of viral replication was determined by immunolabelling of viral antigen using antibodies tagged with horseradish peroxidase. The cytoimmunolabelling method was shown to be 105 times more sensitive for the detection of rotavirus than commercially available ELISA and latex agglutination kits. The cytoimmunolabelling technique was also shown to be successful for the detection of human rotavirus in river and marine waters and wastewater effluents.This publication has 0 references indexed in Scilit: