A new nucleic acid-protein cross-linking reagent
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 152 (3) , 253-257
- https://doi.org/10.1007/bf00693078
Abstract
A new photoactivable reagent is described, which allows the formation of RNA-protein crosslinks via disulfide bridges in combination with mercaptobutyrimidate. The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking. The main advantages of the reagent are the absence of U.V. generated cross-links, since photoactivation is carried out at 360 nm, on one hand and the ease of cleavage of the cross-link by mild reduction (β-mercaptoethanol) on the other.Keywords
This publication has 16 references indexed in Scilit:
- Specific cross-linking of proteins S7 and L4 to ribosomal RNA, by UV irradiation of Escherichia coli ribosomal subunitsMolecular Genetics and Genomics, 1975
- Photo-induced joining of a transfer RNA with its cognate aminoacyl-transfer RNA synthetaseJournal of Molecular Biology, 1974
- ULTRAVIOLET AND γ‐RAY‐INDUCED REACTIONS OF NUCLEIC ACID CONSTITUENTS. REACTIONS OF PURINES WITH AMINESPhotochemistry and Photobiology, 1974
- Photoaffinity labeling of 23 S rRNA inEscherichia coli ribosomes with poly(U)‐coded ethyl 2‐diazomalonyl‐Phe‐tRNAFEBS Letters, 1973
- Reductive methylation: A method for preparing functionally active radioactive ribosomesFEBS Letters, 1973
- A Native Ribonucleoprotein Complex from Escherichia coli RibosomesProceedings of the National Academy of Sciences, 1973
- Photogenerated reagents for biological receptor-site labelingAccounts of Chemical Research, 1972
- Ribosomal ProteinsEuropean Journal of Biochemistry, 1971
- [58] Synthesis of bacteriophage-specific enzymes directed by DNA in VitroPublished by Elsevier ,1971
- Separation of Large Quantities of Ribosomal Subunits by Zonal UltracentrifugationEuropean Journal of Biochemistry, 1970