Delineation of the regulatory region sequences ofAgrobacterium tumefaciens virB operon
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (12) , 4541-4550
- https://doi.org/10.1093/nar/17.12.4541
Abstract
A virB-lacZ translational fusion was constructed to monitor expression of the Agrobacterium tumefaciens virB operon. Expression of the fusion gene was dependent on the presence of pTiA6 virA, virG, and a plant factor acetosyringone. Analysis of deletion mutants, constructed by exonuclease Bal31 digestion, showed that 68 residues upstream of the virB transcription initiation site was necessary for its expression. A TT .fwdarw. CC substitution of positions -62 and -61 led to a 7 fold reduction in virB expression. The virB upstream region contains a tetradecameric sequence, dPuT/ATDCAATTGHAAPy (D=A, G or T; H=A, C or T), that is conserved in the non-transcribed regions of all vir genes. Alteration of the positoin of this sequence relative to the promoter region sequences had a drastic negative effect on virB expression.This publication has 33 references indexed in Scilit:
- EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitroJournal of Bacteriology, 1988
- Crosstalk between bacterial chemotaxis signal transduction proteins and regulators of transcription of the Ntr regulon: evidence that nitrogen assimilation and chemotaxis are controlled by a common phosphotransfer mechanism.Proceedings of the National Academy of Sciences, 1988
- Characterization of the virB operon from an Agrobacterium tumefaciens Ti plasmid.Journal of Biological Chemistry, 1988
- Conserved domains in bacterial regulatory proteins that respond to environmental stimuliCell, 1987
- Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC.Proceedings of the National Academy of Sciences, 1986
- virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciensCell, 1986
- Promoters ofAgrobacterium tumefaciensTi-plasmid virulence genesNucleic Acids Research, 1986
- Rapid and efficient site-specific mutagenesis without phenotypic selection.Proceedings of the National Academy of Sciences, 1985
- Cyclic AMP Receptor Protein: Role in Transcription ActivationScience, 1984
- Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proceedings of the National Academy of Sciences, 1980