MULTIPLE LEVELS OF REGULATION OF MEGAKARYOCYTOPOIESIS
- 1 January 1989
- journal article
- research article
- Vol. 15 (1) , 123-133
Abstract
A working hypothesis for the regulation of megakaryocytopoiesis is described on the basis of current data. The hypothesis proposes that in vivo megakaryocytes are generated by 1) the expansion of clonable progenitor cells into immature megakaryocytes by locally produced (and regulated) interleukin-3 (IL-3) and 2) the development and maturation of immature megakaryocytes by a dual system; by a lineage specific mechanism involving thrombopoietic stimuli in the steady state and thrombocytopenic conditions, and by a lineage nonspecific mechanism via IL-3 in damaged or reconstituting marrow. The hypothesis predicts that if IL-3 is a significant in vivo regulator of megakaryocyte formation and development, receptor for IL-3 should be present on megakaryocytes and may be vestigially on platelets. Small but significant levels of 125I IL-3 were found to bind to platelets from normal mice. The level of binding on platelets was found to be enhanced sevenfold from mice that had received high levels of irradiation followed by bone marrow transplantation. This contrasted with a twofold increase in the level of binding to platelets from mice made acutely thrombocytopenic with antiplatelet serum. The data suggest that IL-3 may be involved in the in vivo regulation of murine megakaryocytopoiesis and may be a significant factor in rebound thrombopoiesis following bone marrow damage.This publication has 21 references indexed in Scilit:
- Immature megakaryocytes in the mouse: In vitro relationship to megakaryocyte progenitor cells and mature megakaryocytesJournal of Cellular Physiology, 1982
- Kinetic analysis of megakaryocyte numbers and ploidy levels in developing colonies from mouse bone marrow cellsCell Proliferation, 1982
- IMMATURE MEGAKARYOCYTES IN THE MOUSE - PHYSICAL CHARACTERISTICS, CELL-CYCLE STATUS, AND INVITRO RESPONSIVENESS TO THROMBOPOIETIC STIMULATORY FACTOR1982
- THE EFFECTS OF THROMBOPOIETIN ON MEGAKARYOCYTE-CFC, MEGAKARYOCYTES, AND THROMBOPOIESIS - WITH STUDIES OF PLOIDY AND PLATELET SIZE1982
- Megakaryocytopoiesis in the mouse: Response to varying platelet demandJournal of Cellular Physiology, 1981
- CELL-INTERACTIONS INFLUENCING MURINE MARROW MEGAKARYOCYTES - NATURE OF THE POTENTIATOR CELL IN BONE-MARROW1981
- DENSITY SUB-POPULATIONS OF RABBIT PLATELETS - SIZE, PROTEIN, AND SIALIC-ACID CONTENT, AND SPECIFIC RADIOACTIVITY CHANGES FOLLOWING LABELING WITH SULFATE-S-35 INVIVO1981
- REGULATION OF MEGAKARYOPOIESIS IN LONG-TERM MURINE BONE-MARROW CULTURES1978
- STIMULATION OF MEGAKARYOCYTOPOIESIS BY ACUTE THROMBOCYTOPENIA IN RATS1976
- Depression of the Megakaryocyte-Platelet System in Rats by Transfusion of PlateletsActa Haematologica, 1967