Enzymes of Aspergillus Oryzae IV. Fractionation and Preparation of Crystals Rich in Protease

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Abstract
Several methods have been examined for the separation of proteases from other enzymes and metabolic products in the culture filtrates obtained by surface culture of Aspergillus oryzae on sucrose-tartrate liquid medium. Concentration, by low-temperature precipitation with ethanol followed by ethanol fractionation of the dialysed concentrate at pH 4-5 in acetate buffer, is shown to be superior' to adsorption methods or precipitation with flavianic acid or ammonium sulphate.