GLUTATHIONE-DEPENDENT PROTECTION AGAINST OXIDATIVE DAMAGE OF THE HUMAN RED-CELL MEMBRANE

  • 1 January 1984
    • journal article
    • research article
    • Vol. 63  (5) , 1096-1101
Abstract
Glutathione (GSH) dependent protection against oxidative damage of human red cell membrane was examined. An artificial system was used in which chloroform/methanol-extracted red cell lipids, in the form of liposomes, were subjected to attack by a peroxidation system consisting of ascorbate-Fe3+. Human erythrocytes contained a nondialyzable factor, completely inactivated by heating in a boiling water bath for 3 min, which showed GSH-dependent inhibition against lipid peroxidation and was devoid of GSH peroxidase activity. GSH-S transferase, highly purified by affinity chromatography, had no inhibitory activity. The GSH-dependent protection against lipid peroxidation of human red cell membrane is mediated by 1 or more proteins other than GSH peroxidase and GSH-S transferase.

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