Abstract
Second trimester [human] amniotic fluid samples (208) were examined visually and spectrophotometrically for pigmentation. Of these, 15 samples had dark brown coloration. Of the 15 samples, 14 had chemically detectable Hb derived pigments and 1 had meconium only. All 15 of the pigmented amniotic fluid samples had hemoglobin A and 8 also had hemoglobin F. None of the remaining 193 amniotic fluid samples contained abnormal pigmentation. Of the pregnancies from which the dark brown amniotic fluid was obtained, failed to continue to term; in 3 of these myoglobin was detected.

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