A hydrophobic platform as a mechanistically relevant transition state stabilising factor appears to be present in the active centre of all glycoside hydrolases
Open Access
- 19 February 2003
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 538 (1-3) , 1-7
- https://doi.org/10.1016/s0014-5793(03)00148-0
Abstract
An in silico survey of the −1 subsite of all known 3D‐structures of O‐glycoside hydrolases containing a suitably positioned ligand has led to the recognition – apparently without exceptions – of a transition state stabilising hydrophobic platform which is complementary to a crucial hydrophobic patch of the ligand. This platform is family‐specific and highly conserved. A comprehensive list is given with examples of enzymes belonging to 33 different families. Several typical constellations of platform – protein residues are described.Keywords
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