Polymer Retention Mechanism in GPC on Active Gels

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Abstract
The elution behaviour of low molecular weight polystyrene (PS) down to the monomer on spherosil gel in several eluents has been studied. In the medium range of molecular weights the elution behaviour is similar to that followed by high molecular weight PS, the differences in elution volumes among different eluents increasing monotonically with decreasing molecular weight and partition rather than adsorption probably being responsible for the shifts in retention volumes. However, at the total permeation limit, the situation is alike to that found in liquid-solid chromatography, the elution volumes of solutes with molecular sizes very close to that of styrene being determined by solvent strength (∊) and solute solubility (δ) parameters. Hydrogen bonding seems to be the main contribution to substrate-solute interactions in this case.