Human α1 ‐antitrypsin expression in Xenopus oocytes
- 1 December 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 153 (2) , 361-365
- https://doi.org/10.1111/j.1432-1033.1985.tb09311.x
Abstract
Injection of equivalent amounts of normal (PiMM) or abnormal (PiZZ) α1‐antitrypsin mRNA into Xenopus oocytes resulted in secretion of both the normal and abnormal α1‐antitrypsin. A much lower proportion of the abnormal protein was secreted, and the Z α1‐antitrypsin that was not secreted accumulated within the cell in a high‐mannose form. The time taken for secretion of the normal and abnormal proteins was identical. Both the secreted and intracellular α1‐antitrypsin synthesized by oocytes were functionally active.This publication has 20 references indexed in Scilit:
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