Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay
- 1 January 1999
- journal article
- research article
- Published by American Society for Microbiology in Clinical and Diagnostic Laboratory Immunology
- Vol. 6 (1) , 130-132
- https://doi.org/10.1128/cdli.6.1.130-132.1999
Abstract
We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 104bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.Keywords
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