Iron dependent ureides and allantionase synthesis in pigeon pea

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Abstract
The effect of iron (Fe) on ureide metabolism was examined in 45‐day‐old pigeon pea (Cajanus cajan L.) (ureide plant) and alfalfa (Medicago sativa) (amide plant). Plants were either inoculated with Rhizobium or fertilized with ammonium nitrate (NH4NO3). The ureides, allantoin and allantoate, and allanotinase activity were increased in pigeon pea with Fe supplementation. Specific effect of Fe on ureide metabolism of pigeon pea was indicated by the lack of same effect in alfalfa under similar conditions. Nitrogenase activity was elevated with increasing concentrations of Fe in pigeon pea (ureide) as well as alfalfa (amide) symbiosis. Nitrogen (N2) fixation, ureides, and allantoinase activity were reduced at 10 ppm and above concentration of Fe.