Subcommittee on the Taxonomy of Gram-Negative Anaerobic Rods: Minutes of the Meetings, 22 and 23 July 1975 Dr. I. Cantacuzino Institute, Bucharest, Romania

Abstract
The genetic relatedness of some nonmotile, ring-forming bacteria was determined by deoxyribonucleic acid (DNA) base composition and base sequence homology experiments. It is proposed that four gas vacuolate strains with DNA base compositions of 67.1 to 68.8 mol% guanine plus cytosine (G + C) be placed in Microcyclus aquaticus. Nonmotile vibrioid bacteria of 51% G + C (Microcyclus flavus Raj and spirosoma strains 1 and 2) were found to be more closely related to one another than to M. aquaticus, and it is suggested that the taxonomic position of M. flavus be reconsidered in relation to the genus Spirosoma. None of the nonmotile ring-forming strains were found to be related to two Pseudomonadaceae. A motile variant of a gas vacuolated strain of M. aquaticus was isolated, indicating that immotility is a questionable taxonomic criterion for inclusion in the genus Microcyclus.