QUANTITATION OF ADRIAMYCIN CONTENT BY A SENSITIVE IMMUNOCHEMICAL ASSAY

  • 1 July 1988
    • journal article
    • research article
    • Vol. 8  (4) , 549-552
Abstract
An immunoenzymatic method for the quantitation of adriamycin (ADM) content in tissues as well as in tumor cells had been developed. This procedure has three main advantages. Firstly, it is possible to carry out the determination on whole homogenates and blood serum, thus avoiding the extractive procedures. Secondly, very low ADM concentrations (0.2 ng) can be detected. Thirdly, it is possible to determine simultaneously and in triplicate both the standard curve and ADM concentration in twelve different samples with a great reduction of the experimental variability.