Renal protein and ammonia biochemistry in NH4Cl acidosis and after uninephrectomy

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Abstract
A comparison was made between certain aspects of the hyperplastic response in the remaining kidney after uninephrectomy and in the kidney in NH4Cl acidosis. In both types of hyperplasia there is early stimulation of protein synthesis that is blocked by low doses of actinomycin D. In the acidotic animal this substance also inhibits the usually observed increase in renal glutaminase and ammonia excretion. Renal extraction of glutamine is increased in the acidotic rat, but not after uninephrectomy. In this latter condition there is also no change in renal glutaminase or ammonia excretion. Cortex slices from kidneys of acidotic rats show a marked increase in the incorporation of L-glutamine-14C into protein by 6 hr. after NH4Cl, but no change up to 36 hr. with L-leucine-14C. Slices from cortex of remaining kidney after uninephrectomy show just the opposite pattern; here incorporation of L-leucine-14C is increased at 6 hr. while there is no change with respect to L-glutamine-14c.

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