Bildung von Phaseolotoxin und Phosphosulfamylornithin durch Pseudomonas syringae pv. phaseolicola in Submerskultur

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Abstract
Phaseolotoxin (Nδ‐phosphosulfamyl‐ornithyl‐alanyl‐homoarginine) from culture‐supernatants of Pseudomonas syringae pv. phaseolicola and Nδ‐phosphosulfamylornithine (PSOrn) obtained from phaseolotoxin by treatment with leucine aminopeptidase were compared in regard to their inhibition effects by means of the enzymatic toxin test as well as by bioassay. In the enzymatic toxin test PSOrn exhibits an inhibiting effect five times stronger than that of phaseolotoxin. The maximal inhibition due to PSOrn is also higher. On the other hand, only phaseolotoxin caused growth inhibition in the bioassays used (indicator organisms: Escherichia coli, Euglena gracilis). Tests are presented which allow to detect these compound selectively. Their application showed that the strain studied produced phaseolotoxin as well as PSOrn in submerged cultures.