Protoplasts: Preparation from Higher Plants
- 14 October 1966
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 154 (3746) , 280-281
- https://doi.org/10.1126/science.154.3746.280
Abstract
Protoplasts can be readily prepared from higher-plant tissue with a concentrated Myrothecium cellulase preparation. An ionic osmoticum produced a higher yield and more viable protoplasts than did the nonionic mannitol osmoticum. Addition of polyuronidases or of ethylenediaminetetra-acetate did not increase the yield. Protoplasts could be prepared from root, leaf, and callus tissue as well as from coleoptiles.This publication has 8 references indexed in Scilit:
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- Colony Formation by Isolated Convolvulus Cells Plated on Defined MediaPlant Physiology, 1965
- Ion Uptake by Cells Enzymically Isolated from Green Tobacco LeavesPlant Physiology, 1965
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