Immunodiffusion and gel‐electrophoretic studies of human articular cartilage proteoglycan

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Abstract
Human articular cartilage proteoglycan has been prepared by non‐disruptive extraction with 4M guanidine and fractionated by CsCI density gradients. Immunodiffusion studies revealed at least three antigenic components. The proteoglycan subunit (PGS) contains an antigenic component common to proteoglycan of many species. The glycoprotein link fraction (GPL) also contains a species‐specific antigen identical to a component of human synovial fluid and one or more antigens in common with bovine GPL. SDS‐gel electrophoresis of proteoglycan fractions from normal cartilage yielded protein bands within the gels with GPL and a protein band at the gel origin with PGS. The proteoglycan subunit from the single sample of osteoarthritic cartilage studied gave an additional metachromatically staining band within the gel.