Effect of DIDS on renal tubular transport

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Abstract
Two stilbene derivates that had been used to covalently label the Cl- carrier in the erythrocyte were investigated for reactivity with the rabbit renal organic anion system. These compounds, 4,4''-diisothiocyanostilbene-2,2''-disulfonate (DIDS) and (4,4''-diisothiocyano)-dihydrostilbene-2,2''-disulfonate (H2DIDS), were potent inhibitors (Ki .simeq. 35 .mu.M) of p-aminohippurate (PAH) transport in the renal cortical slice without affecting tetraethylammonium (TEA) transport or tissue viability. During renal clearance studies performed in the perfused kidney, DIDS decreased the PAH/inulin clearance ratio to .simeq.1. When the possible renal transport of [3H]H2DIDS was investigated, the renal slice transport or binding of [3H]H2DIDS reached a slice-to-medium ratio of .simeq.6, and this accumulation was decreased by probenecid. In perfused kidney experiments, the [3H]-H2DIDS/inulin clearance ratio was .simeq.0.8. Since probenecid reduced this clearance ratio to .simeq.0.5, there was the possibility that H2DIDS underwent tubular secretion. DIDS and H2DIDS interacted with the renal organic anion transport system, which indicated that these compounds were possible probes for this transport system.