Removal of Follicle Wall Components from Ovarian Oocytes of the Brook Trout, Salvelinus fontinalis

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Abstract
Fully grown brook trout (Salvelinus fontinalis) ovarian follicles, preincubated in Ca2+-free media, were dissected from other ovarian tissue by use of watchmaker forceps. Manual defolliculation and further Ca2+-free treatments were used to obtain oocytes freed of one or more follicular investments. Combinations of these treatments ultimately resulted in oocytes denuded of somatic cells as demonstrated by scanning electron microscopy. Mechanically denuded brook trout oocytes maintained the ability to undergo progestogen-induced meiotic maturation in vitro. This technique, which avoids use of enzymes that may alter cell surfaces, provides a useful system for the study of interactions between follicle wall components and the oocyte during hormonally induced meiotic maturation in vitro.

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