Transfection of Escherichia coli by Mu DNA
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 149 (3) , 323-328
- https://doi.org/10.1007/bf00268534
Abstract
Infectivity of Mu DNA was demonstrated in Ca++-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10-7 per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.This publication has 42 references indexed in Scilit:
- Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonucleaseJournal of Molecular Biology, 1975
- RECOMBINATION DEFICIENT MUTANTS OF E. COLI AND OTHER BACTERIAAnnual Review of Genetics, 1973
- Genetic transformation in E.coli: The inhibitory role of the recBC DNaseBiochemical and Biophysical Research Communications, 1973
- The genetic and biochemical basis of the transformability of Escherichia coli K12Biochemical and Biophysical Research Communications, 1972
- Interaction of the recombination pathways of bacteriophage λ and its host Escherichia coli K12: Effects on exonuclease V activityJournal of Molecular Biology, 1972
- Endonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutantsJournal of Molecular Biology, 1968
- Gene orientation in bacteriophage lambda as determined from the genetic activities of heteroduplex DNA formed in vitroJournal of Molecular Biology, 1968
- Changes in the structure and activity of λ DNA in a superinfected immune bacteriumJournal of Molecular Biology, 1965
- Novel intra-cellular forms of lambda DNAJournal of Molecular Biology, 1964
- The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage λdgJournal of Molecular Biology, 1960