ThephbC(poly-β-hydroxybutyrate synthase) gene ofRhizobium(Sinorhizobium)melilotiand characterization ofphbCmutants
- 1 June 1998
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 44 (6) , 554-564
- https://doi.org/10.1139/w98-033
Abstract
Defined insertion mutations have been constructed in theRhizobium (Sinorhizobium) meliloti phbC gene, which encodes poly-β-hydroxybutyrate (PHB) synthase. The locus was isolated and subcloned from a genomic library of R. meliloti Rm1021 by complementation of a phbC mutation of Alcaligenes eutrophus. PHB production was detected in wild-type R. meliloti under nutrient-limited conditions but not in rich medium. No PHB production was detected in the R. meliloti phbC mutants. The DNA sequence of the R. meliloti phbC gene was determined. The deduced polypeptide sequence is homologous to previously identified PhbCs from other bacteria. The R. meliloti phbC locus maps to pRmeSU47a, the smaller of the two megaplasmids in this strain.Key words: Rhizobium meliloti, PHB, PHA, poly-β-hydroxybutyrate, phbC.Keywords
This publication has 27 references indexed in Scilit:
- Isolation and characterization of mutants of Rhizobium meliloti unable to synthesize poly-β-hydroxybutyrateCanadian Journal of Microbiology, 1994
- Overexpression and Purification of the Soluble Polyhydroxyalkanoate Synthase from Alcaligenes eutrophus: Evidence for a Required Posttranslational Modification for Catalytic ActivityBiochemistry, 1994
- The presence of a novel type of surface polysaccharide in Rhizobium meliloti requires a new fatty acid synthase‐like gene cluster involved in symbiotic nodule developmentMolecular Microbiology, 1993
- Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteriaFEMS Microbiology Letters, 1992
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Fine structural analysis of the Zoogloea ramigera phbA‐phbB locus encoding β‐ketothiolase and acetoacetyl‐CoA reductase: nucleotide sequence of phbBMolecular Microbiology, 1989
- A novel exopolysaccharide can function in place of the Calcofluor-binding exopolysaccharide in nodulation of alfalfa by Rhizobium melilotiCell, 1989
- Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutantsGene, 1982
- Transfer of the drug-resistance transposon Tn5 to RhizobiumNature, 1978
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976