Effects of temperature and SDS on the structure of β-glycosidase from the thermophilic archaeon Sulfolobus solfataricus
- 1 May 1997
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 323 (3) , 833-840
- https://doi.org/10.1042/bj3230833
Abstract
The effects of temperature and SDS on the three-dimensional organization and secondary structure of β-glycosidase from the thermophilic archaeon Sulfolobus solfataricus were investigated by CD, IR spectroscopy and differential scanning calorimetry. CD spectra in the near UV region showed that the detergent caused a remarkable change in the protein tertiary structure, and far-UV CD analysis revealed only a slight effect on secondary structure. Infrared spectroscopy showed that low concentrations of the detergent (up to 0.02%) induced slight changes in the enzyme secondary structure, whereas high concentrations caused the α-helix content to increase at high temperatures and prevented protein aggregation.Keywords
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