Effect of divalent and monovalent cations on calf thymus PCNA‐independent DNA polymerase δ and its 3' → 5' exonuclease
- 1 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 259 (2) , 349-352
- https://doi.org/10.1016/0014-5793(90)80045-k
Abstract
Recent data suggest that DNA polymerases α and δ might have a coordinate functional role at the replication fork. In this communication we show that Mg2+ is likely the natural metal activator for both enzymes. Mn2+, a known mutagenic agent, is a competitive inhibitor of Mg2+ for DNA polymerase δ and acompetitive for DNA polymerase α. The 3'→ 5' exonuclease activity associated with DNA polymerase δ is not affected upon addition of Mn2+, Be2+, another mutagenic agent, on the other hand, has an inhibitory effect on the 3' → 5' exonuclease, but not on the DNA polymerase δ. The data presented might explain the mutagenic and carcinogenic potential of these two divalent cations.Keywords
This publication has 13 references indexed in Scilit:
- Calf thymus DNA polymerase δ Independent of proliferating cell nuclear antigen (PCNA)Nucleic Acids Research, 1989
- Eukaryotic DNA Polymerases and δ: Conserved Properties and Interactions, from Yeast to Mammalian CellsProgress in Nucleic Acid Research and Molecular Biology, 1989
- Mammalian DNA polymerases .alpha. and .delta.: current status in DNA replicationBiochemistry, 1988
- Do DNA polymerases δ and α act coordinately as leading and lagging strand replicases?FEBS Letters, 1988
- Calf thymus DNA polymerase δ: purification, biochemical and functional properties of the enzyme after its separation from DNA polymerase α, a DNA dependent ATPase and proliferating cell nuclear antigenNucleic Acids Research, 1988
- Cyclin (PCNA, auxiliary protein of DNA polymerase δ) is a central component of the pathway(s) leading to DNA replication and cell divisionFEBS Letters, 1987
- A cryptic proofreading 3'----5' exonuclease associated with the polymerase subunit of the DNA polymerase-primase from Drosophila melanogaster.Proceedings of the National Academy of Sciences, 1987
- Mammalian DNA polymerase α: a replication competent holoenzyme form from calf thymusNucleic Acids Research, 1987
- An auxiliary protein for DNA polymerase-delta from fetal calf thymus.Journal of Biological Chemistry, 1986
- Further studies on calf thymus DNA polymerase .delta. purified to homogeneity by a new procedureBiochemistry, 1984