DNA Pooling: a tool for large-scale association studies
Top Cited Papers
- 1 November 2002
- journal article
- review article
- Published by Springer Nature in Nature Reviews Genetics
- Vol. 3 (11) , 862-871
- https://doi.org/10.1038/nrg930
Abstract
DNA pooling is a practical way to reduce the cost of large-scale association studies to identify susceptibility loci for common diseases. Pooling allows allele frequencies in groups of individuals to be measured using far fewer PCR reactions and genotyping assays than are used when genotyping individuals. Here, we discuss recent developments in quantitative genotyping assays and in the design and analysis of pooling studies. Sophisticated pooling designs are being developed that can take account of hidden population stratification, confounders and inter-loci interactions, and that allow the analysis of haplotypes.Keywords
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