Comparison of platelet, leukocyte, and growth factor levels in point‐of‐care platelet‐enriched plasma, prepared using a modified Curasan kit, with preparations received from a local blood bank
- 20 May 2003
- journal article
- research article
- Published by Wiley in Clinical Oral Implants Research
- Vol. 14 (3) , 357-362
- https://doi.org/10.1034/j.1600-0501.2003.00810.x
Abstract
The potential use of autologous thrombocytic growth factors to accelerate bone regeneration requires improved methods of isolating platelet‐rich plasma (PRP). In addition to discontinuous cell separation, a second method by which PRP is produced at the point‐of‐care has now become available. In this study, growth factor levels in PRP from these two sources were compared. Whole blood was drawn from 115 healthy donors (73 males, 42 females) aged 21 – 62 years (mean 36, SD 10). The PRP was separated by the blood bank (BB) using the discontinuous cell separation method or at the ‘point‐of‐care’ by the so‐called ‘buffy coat’ method (analogous to the Curasan PRP Kit). Growth factor content differed significantly for TGF‐β1 (BB 268.65±70.77 ng/ml, Curasan 95.02±60.67 ng/ml (sign test PPP<0.160)) showed no significant difference. The higher thrombocyte count in the BB PRP (BB 1434300±351960/μl, Curasan 908.500±492.30/μl) seems to result in higher TGF‐β1 levels, while the higher leukocyte count in the Curasan PRP (BB 160±320/μl, Curasan 30130±12500/μl) seems to result in higher PDGF‐AB levels. The similar IGF‐I levels in the two preparations might merely reflect similar amounts of plasma in the PRP produced by each approach.Keywords
This publication has 17 references indexed in Scilit:
- Kostenanalyse autologer Transfusionsverfahren - eine Untersuchung bei 5017 PatientenAINS - Anästhesiologie · Intensivmedizin · Notfallmedizin · Schmerztherapie, 1999
- Increased content of epidermal growth factor in platelet lysates in non-insulin-dependent diabetes mellitusLife Sciences, 1993
- Platelet‐derived growth factor and dexamethasone combined with a collagen matrix induce regeneration of the periodontium in monkeysJournal of Clinical Periodontology, 1993
- Effects of the Platelet‐Derived Growth Factor/Insulin‐Like Growth Factor‐I Combination on Bone Regeneration Around Titanium Dental Implants. Results of a Pilot Study in Beagle DogsThe Journal of Periodontology, 1991
- The Effects of Short‐Term Application of a Combination of Platelet‐Derived and Insulin‐Like Growth Factors on Periodontal Wound HealingThe Journal of Periodontology, 1991
- Bone Growth FactorsClinical Orthopaedics and Related Research, 1991
- Cytoskeleton-dependent release of human platelet epidermal growth factorLife Sciences, 1991
- Activity of Platelet‐Derived Growth Factor (PDGF) in Platelet Concentrates and Cryopreserved Platelets Determined by PDGF BioassayVox Sanguinis, 1987
- Tests of equality of dependent correlation coefficientsBiometrika, 1977