Some factors influencing the behaviour of BHK 21 Cl 13 cells in soft agar medium

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Abstract
As part of an attempt to reproduce Styles'' cell transformation assay, the effect of serum concentration on the growth of normal and 4-nitroquinoline-1-oxide (4-NQO)-treated BHK 21 Cl 13 cells in soft agar medium was examined. Using medium with 10% newborn calf serum, dilution of control cultures from 5 .times. 104 to 1.56 .times. 103 cells/ml caused little increase in the number of countable (> 0.3 mm diameter) colonies, but 4NQO caused a marked dose-related increase. Using 20% of the same batch of serum, 4NQO-treated groups and controls diluted to comparable viability counts showed very similar increases in countable colonies. Clones > 0.3 mm in diameter isolated from control cultures with 20% serum did not appear to be transformed when grown in agar with 10% serum. Factors other than neoplastic transformation can influence the growth of BHK 21 Cl 13 cells in soft agar medium.