Identification of sequences regulating the transcription of aDictyostcliumgene selectively expressed in prespore cells
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (16) , 6473-6484
- https://doi.org/10.1093/nar/17.16.6473
Abstract
There has been considerable debate about the relative contributions of transcriptional and post-transcriptional mechanisms to the regulation of prespore gene expression in Dictyostelium. We have determined the DNA sequence upstream of D19, the Dictyostelium gene encoding PsA, a prespore-specific, cell surface protein of unknown function. Our analysis of gene fusions, in which D19 upstream sequences are placed adjacent to a heterologous reporter gene, indicates that transcriptional signals alone are sufficient for the correct temporal and cell-type specific expression of this gene. We also show that the 5'' and 3'' boundaries of the minimals sequences necessary for correct developmental regulation lie within the the region 338 to 122 nucleotides upstream of the start site of transcription but that flanking sequences seem to be necessary for optimal expression.This publication has 33 references indexed in Scilit:
- Characterization of two divergently transcribed Dictyostelium gene pairs and identification of G‐rich sequence element lying between them with the characteristics of a basal promoter elementDevelopmental Genetics, 1988
- Two vectors which facilitate gene manipulation and a simplified transformation procedure for Dictyostelium discoideumGene, 1987
- Postaggregative differentiation induction by cyclic AMP in Dictyostelium: Intracellular transduction pathway and requirement for additional stimuliDevelopmental Biology, 1986
- Changes during differentiation in requirements for cAMP for expression of cell-type-specific mRNAs in the cellular slime mold, Dictyostelium discoideumDevelopmental Biology, 1986
- Conserved structural features are found upstream from the three co-ordinately regulated discoidin I genes of Dictyostelium discoideumJournal of Molecular Biology, 1984
- Cyclic AMP stabilizes a class of developmentally regulated Dictyostelium discoideum mRNAsNature, 1983
- Messenger RNA half-life in Dictyostelium discoideumDevelopmental Biology, 1983
- Differences in the stability of developmentally regulated mRNAs in aggregated and disaggregated Dictyostelium discoideum cellsDevelopmental Biology, 1982
- Separation and properties of prestalk and prespore cells of Dictyostelium discoideumExperimental Cell Research, 1981
- The role of cyclic nucleotides and cell agglomeration in postaggregative enzyme synthesis in Dictyostelium discoideumDevelopmental Biology, 1978