In-vitro Study of DNA-Synthesis Time and Cell-Cycle Time in Erythrocyte Precursors of Normal and Thalassaemic Subjects, Using a 3H- and 14C-Thymidine Double Labelling Technique

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Abstract
Bone marrow cells from normal subjects and thalassaemic patients were incubated for 1 hr. with 3H-thymidine and then 14C-thymidine was added and incubation continued for 10 min. Smears were prepared for autoradiography and a double emulsion technique was followed. The 1st emulsion registered both 3H- and 14c-beta particles and the 2nd 14C-beta particles only. It was thus possible to distinguish between cells containing 3H-thymidine only and cells containing both 3H- and 14C-thymidine. From the percentages of labelled cells of each kind the DNA synthesis time and the cell cycle time were calculated. Both were shorter than normal in thalassemic erythroid precursors.