Use of Guinea Pig Eye in Study of Intraocular Infections Produced by Mumps Virus.

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Abstract
Mixtures of mumps virus with either normal pooled human sera (collected from mumps-susceptible children, 2.5-5 yrs. old) or specific pooled mumps immune sera were injected into replicates of guinea pigs'' eyes. Mumps immune sera neutralized the mumps virus and completely prevented the corneal reaction and the appearance of complement-fixing antibodies in the convalescent sera of the guinea pigs. Normal pooled human sera showed a definite antiviral effect by preventing and modifying the corneal reaction as well as modifying the titer of complement fixing antibodies against the mumps virus in the convalescent sera of guinea pigs. Intraocular injn. of mumps virus in guinea pigs produced the characteristic corneal reactions of the usual duration. High titers of complement fixing antibodies appeared uniformly in the convalescent sera. Between the 4th and 8th day after the intraocular injn. of mumps virus in guinea pigs, complement-fixing antibodies appear in the convalescent sera. These antibodies reached a peak titer on the 12th day, persisted at this level until the 20th to the 24th day, and then declined. Virus assays in chick embryos demonstrated that viable mumps virus persisted in guinea pig ocular tissue for 8 days after intraocular injn. of virus. Strains of mumps virus recently isolated from human beings produce reactions in guinea pigs that do not differ from those produced by known laboratory strains of mumps virus.
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