BIOCHEMICAL STUDIES ON THE FORMATION OF THE SILKPROTEIN

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Abstract
After the injection of phenylalanine-2-C14 to silk worm (Si 122 x Nichi 122) on the 4th day at 5th instar in an amount of 0.5 [mu]C/larva), the specific radioactivies of sericin and fibroin separated from cocoon fibers were 1407 and 4531 cpm/100 mg, respectively. Among amino acids composing fibroin, tyrosine possessed exclusively high activity (41.147 cpm/[mu]mol.). This was also proved by paper chromatography with a phenol-0.1% NH3 mixture (85:15), which followed by radioautogram as well as by radioactivity estimation with a SC-16 gas flow windowless counter.