Intracellular distribution of DNA methyltransferase during the cell cycle
- 15 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 236 (1) , 9-13
- https://doi.org/10.1016/0014-5793(88)80275-8
Abstract
The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nuclei with weak cytoplasmic staining. The DNA methyltransferase antigen was absent in early G1 phase, appeared in late G1 prior to the onset of DNA synthesis and persisted throughout S and G2 phases of the cell cycle. Mitotic cells showed a particularly strong staining intensity. These results show that DNA methyltransferase levels fluctuate during the cell cycle. This has possible implications on the stability of the DNA methylation pattern.Keywords
This publication has 15 references indexed in Scilit:
- Differential expression of DNA topoisomerases I and II during the eukaryotic cell cycle.Proceedings of the National Academy of Sciences, 1988
- DNA methylases separated through the HeLa cell cycle methodology show allosteric propertiesFEBS Letters, 1987
- DNA methyltransferase polypeptides in mouse and human cellsBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1986
- Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replicationExperimental Cell Research, 1986
- Identification of Proliferatively Active Cells by Monoclonal Antibodies Against Dna-Cytosine-5 MethyltransferasePublished by Elsevier ,1985
- Delayed methylation and the matrix bound DNA methylaseBiochemical and Biophysical Research Communications, 1985
- Intracellular localization and metabolism of DNA polymerase α in human cells visualized with monoclonal antibodyExperimental Cell Research, 1984
- DNA METHYLATION AND GENE ACTIVITYAnnual Review of Biochemistry, 1983
- DNA-methylase from regenerating rat liver: purification and characterisationNucleic Acids Research, 1978
- Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cellsExperimental Cell Research, 1963