Evidence for synthesis in vitro of neutral lipids by isolated oocytes ofPerinereis cultrifera(Annelida, Polychaeta). A biochemical and autoradiographic study

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Abstract
Isolated oocytes of Perinereis cultrifera have been incubated in culture media with added [3H]glycerol, [14C]butyric acid or [14C]oleic acid. The principal neutral lipid synthesized was triacylglycerol, although incorporation of radioactivity into other lipid categories (sterol, fatty acid, wax ester) was also observed. A more significant percentage of triacylglycerol was labelled after incubation with [3H]glycerol and [14C]oleic acid than with [14C]butyric acid. With this precursor, monoacylglycerol appears to be the class of lipid compartment which initially show the most radioactivity. Electron microscopic autoradiography has revealed that labelling after incorporation of glycerol was mainly localized on the lipid droplets but not on the yolk granules. A second metabolic pathway is represented by phospholipid membrane synthesis.