Evaluation of a static renewal chronic toxicity test method for Daphnia magna straus using boric acid

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Abstract
A procedure for conducting chronic daphnid static renewal tests has been developed and evaluated, using boric acid as the test material. This procedure is an improvement over other proposed procedures for the following reasons: (a) the test methodology and equipment simplify the operations required to perform the test, (b) data on all endpoints for a particular concentration are obtained from the same group of test organisms and (c) the experimental design permits statistical interpretation of the critical endpoints. The acute and chronic effects of boric acid on Daphnia magna were similar to those reported in the literature. The 48‐h static acute LC50 value (and 95% confidence intervals) for boric acid were 133 (115–153) mg/L as boron. The chronic toxicity of boric acid estimated by derivation of the maximum acceptable toxicant concentration was between 6.4 and 13.6 mg/L as boron.