The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro
- 1 April 1987
- journal article
- Published by Springer Nature in Nature
- Vol. 326 (6112) , 471-475
- https://doi.org/10.1038/326471a0
Abstract
Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr approximately 36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.Keywords
This publication has 35 references indexed in Scilit:
- S‐phase patterns of cyclin (PCNA) antigen staining resemble topographical patterns of DNA synthesisFEBS Letters, 1985
- The termination region for SV40 DNA replication directs the mode of separation for the two sibling moleculesCell, 1985
- Identity of the proliferating cell nuclear antigen and cyclinNature, 1984
- Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera.The Journal of Experimental Medicine, 1984
- Enzymology of DNA in Replication in ProkaryoteCritical Reviews in Biochemistry, 1984
- T antigen repression of SV40 early transcription from two promotersCell, 1981
- T antigen binding and the control of SV40 gene expressionCell, 1981
- Arrest of segregation leads to accumulation of highly intertwined catenated dimers: Dissection of the final stages of SV40 DNA replicationCell, 1981
- SV40 gene expression is modulated by the cooperative binding of T antigen to DNACell, 1981
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976